2024-12-19
Tianlong's ASFV Fluorescent PCR Kit Shows Superior Sensitivity in Comparative Testing
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["title"] => string(85) "Tianlong's ASFV Fluorescent PCR Kit Shows Superior Sensitivity in Comparative Testing"
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["content"] => string(7807) "<p>Tianlong, a leading provider of diagnostic solutions, has completed an extensive comparative evaluation of its African Swine Fever Virus (ASFV) Nucleic Acid Detection Kit (Fluorescence PCR Method) with a competitor's ASFV detection kit. The study assessed several critical performance parameters, including linearity, precision, sensitivity, and accuracy.</p><p>The comparison was carried out using Tianlong's high-performance products, including the Tianlong Animal Virus DNA and RNA Extraction Kit and Gentier 96E Real-Time PCR System, as well as the a competitor's Analyzer. The tested parameters were analyzed across a range of concentrations and sample types, focusing on ASFV (African Swine Fever Virus) detection.</p><p><br/></p><ul style="list-style-type: disc;"><li><p><span style="font-size: 18px;"><strong>Key Findings</strong></span></p></li></ul><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241219/1734585465711267.png" title="" alt="a.png" width="700" height="" border="0" vspace="0" style="width: 700px;"/></p><p><strong>1. Linearity Test: </strong></p><p>Both Tianlong and the competitor's kits demonstrated excellent linearity, with R2 values exceeding 0.98, indicating a strong correlation between the measured and expected results. This suggests that both kits provide consistent and reliable amplification across a wide range of concentrations.</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241219/1734585480780659.png" title="" alt="b.png" width="700" height="" border="0" vspace="0" style="width: 700px;"/></p><p><strong>2. Precision Test: </strong></p><p>The results showed comparable precision between the two kits. Tianlong’s kit demonstrated superior consistency in fluorescence amplification curves, with a lower coefficient of variation (CV) at both high and low concentrations, highlighting its reliable performance in routine testing.</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241219/1734585497656490.png" title="" alt="c.png" width="700" height="" border="0" vspace="0" style="width: 700px;"/></p><p><strong>3. Sensitivity Test: </strong></p><p>In terms of sensitivity, Tianlong's ASFV PCR kit demonstrated a significantly higher detection rate for low-concentration samples (100% detection rate) compared to the competitor's kit (95%). This suggests that Tianlong’s kit is more sensitive and better suited for detecting ASFV at lower concentrations, a crucial advantage in field diagnostics.</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241219/1734585511858416.png" title="" alt="d.png" width="700" height="" border="0" vspace="0" style="width: 700px;"/></p><p><strong>Accuracy Test: </strong></p><p>The accuracy results showed complete concordance between Tianlong and the competitor's kits when testing real-world ASFV-positive samples from various sources, including environmental and biological specimens. This confirms the robustness and reliability of Tianlong’s kit in diverse testing conditions.</p><p><br/></p><ul style="list-style-type: disc;"><li><p><span style="font-size: 18px;"><strong>Key Facts About African Swine Fever (ASF)</strong></span></p><p><br/></p></li></ul><p>Responsible for massive losses in pig populations and drastic economic consequences, African swine fever (ASF) has become a major crisis for the pork industry in recent years. Currently affecting several regions around the world, the disease is not only impeding animal health and welfare but has also detrimental impacts on biodiversity and the livelihoods of farmers. The World Organisation for Animal Health (WOAH) works with its partners, industry and experts to support countries in their efforts to prevent and control this devastating pig disease.</p><p> </p><p><strong>1. What is ASF?</strong></p><p>African Swine Fever (ASF) is a highly contagious viral disease that affects domestic and wild pigs. It is caused by the African Swine Fever Virus (ASFV) and can result in severe hemorrhagic fever in pigs, with nearly 100% mortality in infected animals. ASF does not affect humans or other animal species.</p><p><strong>2. How is ASF Transmitted?</strong></p><p>ASF is primarily transmitted through direct contact with infected pigs, contaminated feed, water, equipment, and even personnel. The virus can also be spread through wild boar populations, making it difficult to control in regions with significant wild pig activity.</p><p><strong>3. Economic Impact:</strong></p><p>ASF has devastating economic consequences for the pig farming industry, as infected herds must be culled to prevent further spread. The disease has caused widespread outbreaks globally, leading to significant losses in pork production and trade disruptions.</p><p><strong>4. Symptoms of ASF:</strong></p><p>Infected pigs may show a range of symptoms, including fever, loss of appetite, skin reddening or blotching, and hemorrhaging. In severe cases, death can occur within a few days of infection.</p><p><strong>5. Prevention and Control:</strong></p><p>Currently, there is no vaccine for ASF, and prevention largely relies on biosecurity measures, including controlling the movement of animals, disinfecting equipment, and ensuring strict quarantine measures in affected areas. Early detection through reliable diagnostic methods is key to containing outbreaks before they spread further.</p><p> </p><ul style="list-style-type: disc;"><li><p><span style="font-size: 18px;"><strong>About Tianlong’s ASFV Nucleic Acid Detection Kit</strong></span></p></li></ul><p style="text-align: center;"><strong><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241219/1734585534891617.png" title="" alt="微信图片_20240611155757.png" width="500" height="" border="0" vspace="0" style="width: 500px;"/></strong></p><p><a href="https://www.medtl.net/products/african-swine-fever-virus-asfv-nucleic-acid-detection-kit-fluorescence-pcr-method.html" target="_self">Tianlong’s ASFV PCR Detection Kit</a> offers wide coverage, detecting both ASFV genotypes I and II. It is highly versatile, suitable for various specimens including blood, serum, tissue, and environmental samples. The kit includes an internal control to prevent false negatives, is user-friendly with compatibility for FAM and VIC (HEX) channels, and ensures high precision with a coefficient of variation (CV%) under 5%.</p><p> </p><ul style="list-style-type: disc;"><li><p><span style="font-size: 18px;"><strong>Conclusion</strong></span></p><p><span style="font-size: 18px;"></span></p></li></ul><p><span style="font-size: 18px;"></span></p><p><span style="font-size: 18px;"></span></p><p><span style="font-size: 18px;"></span></p><p>The comparative testing reinforces the high performance of Tianlong's ASFV Fluorescent PCR Detection Kit, particularly in terms of its superior sensitivity for low-concentration samples. While both kits performed similarly in linearity, precision, and accuracy tests, Tianlong’s superior sensitivity positions its product as an optimal choice for ASFV diagnostics, ensuring higher detection rates in critical applications.</p><p>For the agriculture and veterinary industries, timely and accurate diagnosis of ASF is crucial for controlling outbreaks and minimizing economic damage. Tianlong’s ASFV Fluorescent PCR Detection Kit is a vital tool in the fight against ASF, helping farmers, researchers, and authorities prevent the spread of this devastating disease.</p>"
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