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2024-12-27Personalized Medication: How Ligation Sequencing Solutions Drive Innovation
array(34) { ["id"] => string(3) "699" ["catid"] => string(3) "498" ["title"] => string(75) "Personalized Medication: How Ligation Sequencing Solutions Drive Innovation" ["thumb"] => array(2) { ["thumb"] => string(48) "/Public/Uploads/uploadfile/images/20241227/2.jpg" ["alt"] => string(5) "2.jpg" } ["keywords"] => string(0) "" ["description"] => string(0) "" ["content"] => string(10215) "<p>Medical advancements have profoundly transformed how diseases are diagnosed and treated, yet one principle has remained consistent for much of history—using the same solutions for the same conditions.</p><p>However, with growing insights into human genetics and disease mechanisms, the era of "one-size-fits-all" is being gradually replaced by a more targeted approach, <a href="https://www.medtl.net/products/reagents/ligation-sequencing-kits-precision-medicine" target="_self">personalized medication.</a></p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241227/1735289981896739.png" title="" alt="1.png"/></p><p></p><p style="text-align: center;"><span style="font-size: 12px;">(Copyright Photo from: https://unsplash.com/photos/yellow-medication-pill-on-persons-hand-nj9tmN-7YMA)</span></p><p><strong>What is Personalized Medication?</strong></p><p>Personalized medication, also known as precision medicine or individualized medicine, recognizes that each person responds differently to medications due to their unique biological factors. By tailoring treatments to these specific characteristics, personalized medicine ensures better therapeutic outcomes and fewer adverse reactions.</p><p><strong>The Evolution of Personalized Medication</strong></p><p>Traditionally, medical treatments have often relied on a generalized approach—a practice where identical treatments are given to all patients presenting with similar symptoms or conditions. While effective in some cases, this approach has limitations, as it does not account for the diversity in individual responses to treatment.</p><p><strong>Several advancements have catalyzed the move toward personalized medication:</strong></p><p><strong>1. Advancements in Genomics</strong></p><p>The development of genomics has directly influenced personalized treatment tailoring. Genomic sequencing provides insights into the genetic variations that can affect how individuals metabolize drugs, making it possible to design more effective and precise therapies. For example, pharmacogenomics helps identify how specific genetic markers influence a patient's response to certain medications, guiding optimal drug selection and dosage.</p><p><strong>2. Deeper Understanding of Disease Mechanisms </strong></p><p>Advances in medical research have provided a more nuanced understanding of diseases, highlighting that even common conditions like diabetes or heart disease have subtypes influenced by genetic, environmental, and lifestyle factors. Recognizing these distinctions allows for targeted therapeutic interventions that can address the specific subtypes of a condition.</p><p>Together, genomics and a deeper understanding of disease mechanisms have not only strengthened the foundation for personalized precision medicine but also redefined treatment paradigms across healthcare.</p><p><strong>Applications of Personalized Medication</strong></p><p>The adoption of personalized medication has had significant implications for several aspects of healthcare. Below are its key applications across the field:</p><p><strong>1. Drug Development and Usage</strong></p><p>Pharmaceutical companies are increasingly leveraging personalized medication during drug development. By integrating genomic data into clinical trials, they can identify subpopulations of patients who are most likely to benefit from a specific medication.</p><p>Additionally, doctors and healthcare professionals are using patient-specific data to determine the most appropriate medicines or customize dosages to reduce side effects.</p><p><strong>2. Diagnosis and Intervention</strong></p><p>Personalized medication approaches have also improved diagnostic practices. Opting for molecular diagnostics, for instance, allows for identifying not just the disease itself but also the specific genetic or molecular markers driving it. Especially in the case of <a href="https://www.medtl.net/news/blog/aspirin-resistance-testing-reasons-and-methods.html" target="_self">aspirin resistance</a>, molecular testing can identify patients who do not respond effectively to aspirin therapy, commonly used to prevent cardiovascular events.</p><p>This can enable earlier and more precise medication plans, which is particularly important in areas like oncology. Tailored treatments based on tumor genetic profiling have yielded improved outcomes for patients with certain types of cancers.</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241227/1735290023238663.jpg" title="" alt="2.jpg"/></p><p></p><p style="text-align: center;"><span style="font-size: 12px;">(Copyright Photo from: https://unsplash.com/photos/white-blue-and-orange-medication-pill-KltoLK6Mk-g)</span></p><p><strong>Pros and Cons of Personalized Medication</strong></p><p>While personalized medication presents clear benefits, it also comes with its own set of challenges.</p><p><strong>Pros</strong></p><ul style="list-style-type: disc;"><li><p>Enhanced Efficacy: Personalized medication can be tailored for increased effectiveness, maximizing therapeutic benefits for individual patients. </p></li><li><p>Reduced Adverse Reactions: Understanding a patient’s genetic makeup helps avoid medications that may cause significant side effects. </p></li><li><p>Preventive Potential: Predictive tools can identify the likelihood of developing certain medical conditions, enabling preventive strategies.</p></li></ul><p><strong>Cons</strong></p><ul style="list-style-type: disc;"><li><p>Complexity of Implementation: Establishing tailored regimens requires specialized expertise, extensive data collection, and advanced testing tools. </p></li><li><p>Limited Accessibility: Not all populations have equal access to the infrastructure and technologies needed for personalized medicine, potentially leading to healthcare disparities.</p></li></ul><p><strong>The Role of Ligation Sequencing Kits in Facilitating Personalized Medication</strong></p><p>One of the essential tools in advancing personalized medication is ligation sequencing kits. Ligation sequencing is a DNA sequencing method that involves adding a series of specific DNA sequences (called adaptors) to the ends of DNA fragments, followed by the use of a ligase enzyme to link them together. This technique allows enables precise and efficient identification of genetic variations, making it a critical tool for advancing personalized medication.Adopting ligation sequencing kits into personalized medication can facilitate precise genotyping and molecular analysis, enabling healthcare professionals to make appropriate diagnosis tailored to each patient.</p><p>Ligation sequencing focuses on identifying and analyzing key genetic sequences that affect patient responses to medication. By leveraging this technology, researchers and healthcare providers can:</p><ul style="list-style-type: disc;"><li><p>Detect genetic markers associated with disease risk or treatment resistance. </p></li><li><p>Enhance the accuracy and speed of diagnostics and intervention planning. </p></li><li><p>Optimize drug development pipelines by providing detailed molecular insights.</p></li></ul><p>These kits are especially beneficial when accurate and reliable data are required to customize medication plans for individual patients, making them an indispensable tool in the modern healthcare landscape.</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241227/1735290187760908.png" title="" alt="3.png"/></p><p></p><p><strong>Introducing Tianlong’s Ligation Sequencing Kits</strong> </p><p>At <a href="https://www.medtl.net/" target="_self">Tianlong</a>, we have been a pioneer in nucleic acid testing in China since 1997. Tianlong's <a href="https://www.medtl.net/products/reagents/ligation-sequencing-kits-precision-medicine_2" target="_self">ligation sequencing kits </a>are tailored to provide precise genetic analysis, enabling clinicians to design effective individualized treatment plans. </p><p>In Tianlong’s solution, we can provide various ligation sequencing kits based on your demands, including:</p><ul style="list-style-type: disc;"><li><p>Aspirin Personalized Medication Solutions: Identifying genetic resistance to aspirin.</p></li><li><p>Statins Personalized Medication Solutions: Optimizing cholesterol management based on genetic profiles.</p></li><li><p>Warfarin Personalized Medication Solutions: Supporting safe and effective anticoagulation therapy.</p></li><li><p>Folic Acid Personalized Medication Solutions: Detecting genetic variants related to folic acid metabolism.</p></li></ul><p>These Ligation Sequencing Kits offer an efficient solution for personalized medication by combining reliable detection, easy operation, and high efficiency. They ensure the precise identification of genetic variations, which is critical for individualized treatment plans. Their structure simplifies the sequencing process, allowing broader adoption in clinical and research environments. Additionally, the kits enable accurate and timely diagnostics, making them an essential tool in advancing personalized medicine.</p><p><strong>Conclusion</strong></p><p>Personalized medication represents a shift towards a future where healthcare is built around the individual rather than the disease. By tailoring treatments to each patient's genetic makeup and personal circumstances, we can unlock improved outcomes and a higher standard of care.</p><p>To fully realize its potential, reliable testing tools like Tianlong's ligation sequencing kits will play a crucial role. With decades of expertise and innovative solutions, Tianlong is committed to supporting healthcare providers in implementing cutting-edge approaches to personalized medicine.</p><p>To learn more about Tianlong’s ligation sequencing kits and how they can support your personalized medication initiatives, <a href="https://www.medtl.net/contact" target="_self">reach out to Tianlong today!</a></p>" ["url"] => string(90) "/news/blog/personalized-medication-how-ligation-sequencing-solutions-drive-innovation.html" ["count"] => string(2) "50" ["sort"] => string(1) "0" ["command"] => string(1) "0" ["isdel"] => string(1) "0" ["free"] => string(1) "0" ["status"] => string(1) "1" ["uid"] => string(1) "4" ["username"] => string(11) "system-2022" ["euid"] => string(1) "0" ["edituser"] => string(0) "" ["input_time"] => string(10) "1735289939" ["create_time"] => string(10) "1735290266" ["update_time"] => NULL ["static_title"] => string(0) "" ["tag"] => NULL ["out_url"] => string(0) "" ["custom_code"] => string(0) "" ["jie"] => string(121) "To fully realize its potential, reliable testing tools like Tianlong's ligation sequencing kits will play a crucial role." ["is_ding"] => string(1) "0" ["zname"] => string(0) "" ["index_img"] => array(2) { ["thumb"] => string(52) "/Public/Uploads/uploadfile/images/20241227/2-618.jpg" ["alt"] => string(9) "2-618.jpg" } ["vid"] => string(0) "" ["command_detail"] => string(1) "0" ["title_url"] => string(1) "1" ["url_title"] => string(1) "1" ["iframe"] => string(0) "" }
To fully realize its potential, reliable testing tools like Tianlong's ligation sequencing kits will play a crucial role.
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2024-10-31Aspirin Resistance Testing: Reasons and Methods
array(34) { ["id"] => string(3) "681" ["catid"] => string(3) "498" ["title"] => string(47) "Aspirin Resistance Testing: Reasons and Methods" ["thumb"] => array(2) { ["thumb"] => string(48) "/Public/Uploads/uploadfile/images/20241031/3.png" ["alt"] => string(5) "3.png" } ["keywords"] => string(0) "" ["description"] => string(0) "" ["content"] => string(9827) "<p>Aspirin is widely considered the best practice for antiplatelet therapy in cardiovascular diseases (CVD) management. It is a cornerstone in preventing myocardial infarction by inhibiting platelets from forming blood clots. </p><p>However, the prevalence of aspirin resistance differs greatly across various groups of people based on factors such as gender, age, underlying health conditions, and <a href="https://www.medtl.net/products/aspirin-personalized-medication-solutions.html" target="_self">aspirin resistance testing</a> methodologies.</p><p>A systematic review found that aspirin resistance could be higher in women (26.9%) than men (23.5%)[1], and elderly patients with coronary artery disease had 9.3% to 24.8% aspirin resistance[1].</p><p>These findings underscore the need for accurate aspirin resistance testing to enhance personalized medicine and improve patient outcomes.</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241031/1730352081636463.png" title="" alt="1.png"/></p><p></p><p><strong>How Does Aspirin Inhibit Platelet Aggregation?</strong> </p><p>Aspirin, also known as acetylsalicylic acid, stops platelets from sticking together and forming clots by irreversibly blocking the enzyme cyclooxygenase (COX-1). Platelets need this enzyme to make thromboxane A2, a potent platelet activator, which induces platelet aggregation.</p><p>Without thromboxane A2, platelets can't clump together to form harmful blood clots. Moreover, they have no nucleus and DNA, so no new COX-1 can be produced. Hence, the effect of aspirin on platelets persists until new platelets are made, which takes about 7 to 10 days.</p><p>Even after stopping aspirin, the body may still take some time to resume normal blood clotting. Aspirin also changes a specific enzyme called COX-2. It is required in the prostaglandin pathway to reduce inflammation in the body. This is how aspirin helps decrease pain and swelling when taken in higher doses.</p><p><strong>Mechanisms Behind Aspirin Resistance and Its Clinical Consequences</strong> </p><p>However, this drug can fail to hit its pharmacological target (COX-1), not effectively inhibiting platelet function and clot formation in a certain kind of group. In clinics, it is known as aspirin resistance.</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241031/1730352126721654.png" title="" alt="2.png"/></p><p></p><p style="text-align: center;"><em><span style="font-size: 12px;">(Copyright photo from: https://en.wikipedia.org/wiki/File:Blood_clot_diagram.png)</span></em></p><p><strong>1. Mechanisms of Aspirin Resistance</strong></p><p>To optimize patient management, it's important to understand these mechanisms. Various reasons could cause the occurrence of aspirin resistance, including:</p><p></p><ul style="list-style-type: disc;"><li><p><strong>Reduced Bioavailability</strong> </p></li></ul><p>Aspirin works best when it is absorbed well in the stomach. Factors like pH level and meal intake can affect its absorption rate. Variability in absorption can result in varying plasma concentration levels, which might make it less effective.</p><ul style="list-style-type: disc;"><li><p><strong>Increased Platelet Turnover</strong></p></li></ul><p>In some inflammatory processes, such as atherosclerosis and other complications, the body makes new platelets faster than normal. This reduces aspirin's ability to block COX-1, which results in less efficacy of the drug over time.</p><ul style="list-style-type: disc;"><li><p><strong>Negative Drug Interactions</strong></p></li></ul><p>Taking other drugs such as NSAIDs (non-steroidal anti-inflammatory drugs) along with aspirin can interfere with its binding to COX-1, diminishing its effectiveness in preventing blood clots.</p><p><strong>2. Clinical Consequences of Aspirin Resistance</strong></p><p>A research study has shown that aspirin resistance can raise heart events to 13 times higher in cardiovascular patients[2]. Generally, factors can result in severe clinical consequences:</p><ul style="list-style-type: disc;"><li><p>High risk of myocardial infarction and stroke</p></li><li><p>Alternative antiplatelet agents</p></li><li><p>Difficulty in patient management</p></li><li><p>Need for precise aspirin resistance testing</p></li></ul><p><strong>Methods of Aspirin Resistance Testing</strong></p><p>Accurate aspirin resistance testing is essential for optimizing patient outcomes, as it enables healthcare professionals to personalize aspirin therapy. The following aspirin resistance testing methods are widely used to check its effectiveness:</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241031/1730352173340488.png" title="" alt="3.png"/></p><p></p><p style="text-align: center;"><span style="font-size: 12px;"><em>(Copyright photo from: https://unsplash.com/photos/red-plastic-container-with-white-powder-kQrBavaF-Fk)</em></span></p><p><strong>1. Platelet Function Tests</strong></p><p>Platelet function test is one of the methods to undergo aspirin resistance testing in labs and clinical settings, such as:</p><ul style="list-style-type: disc;"><li><p>Light Transmission Aggregometry (LTA)</p></li><li><p>Platelet Function Analyzer 100/200 (PFA 100/200)</p></li><li><p>Thromboelastography (TEG)</p></li><li><p>Thromboxane B2 (TXB2) Testing </p></li></ul><p>These aspirin resistance tests can be used to measure the efficacy of aspirin in preventing platelet aggregation. However, in a study of 201 patients with stable coronary artery disease, researchers found unclear results in these different tests[3]. Yield significantly varied results when detecting aspirin resistance. It suggests that these tests may not reliably assess aspirin's antiplatelet effects.</p><p>Besides, the study found poor correlation and agreement among the different testing methods as well[3]. This indicates that even when multiple aspirin resistance tests are employed, the conclusions might be inconsistent, complicating clinical decision-making.</p><p><strong>2. Genetic Tests</strong></p><p>Genetic testing for aspirin resistance is an emerging area of research that aims to enhance the effectiveness of aspirin therapy in preventing cardiovascular diseases. Several studies have identified specific genetic variants associated with aspirin resistance, notably including[4]:</p><ul style="list-style-type: disc;"><li><p>ITGB3</p></li><li><p>ITGA2</p></li><li><p>PTGS1</p></li><li><p>PTGS2</p></li></ul><p>Similarly, a study reviewed various laboratory genetic tests for aspirin resistance, showing a strong link between the particular genetic variant “P1A1/A2” in the GPIIIa receptor gene (ITGB3) and aspirin resistance[5].</p><p>Consequently, genetic testing is a more reliable way to identify patients likely to benefit from alternative therapies or adjusted dosages, thereby improving precision medicine and treatment outcomes</p><p><strong>Tianlong’s Aspirin Personalized Medication Solutions</strong></p><p>This association is especially noted in healthy individuals, but it can only be identified if the results are accurate. To ensure error-free results, Tialong’s <a href="https://www.medtl.net/products/reagents/ligation-sequencing-kits-precision-medicine" target="_self">ligation sequencing kit</a>, the Aspirin Personalized Medication Solution, is specifically designed for precision in aspirin resistance testing and subsequent medication use. The features include:</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241031/1730352272828666.png" title="" alt="4.png"/></p><p></p><ul style="list-style-type: disc;"><li><p>99% accuracy in detection results</p></li><li><p>70 minutes process to determine the presence of TGB3 (c.176 T>C), LTC4S (c.-444 A>C), PEAR1 (C.-9-3996 G>A), PTGS1 (c.-842 A>G) and GP1BA (c.482 C>T) </p></li><li><p>Pre-filled reagents without sample extraction</p></li></ul><p><strong>Conclusion</strong></p><p>Aspirin resistance poses a significant challenge for cardiovascular patients. Accurate aspirin resistance testing methods are required for precision medicine. Using accurate and efficient testing reagents like Tianlong’s ligation sequencing kits on aspirin resistance, doctors can offer customized treatments for better safety and aspirin efficacy.</p><p>At Tianlong, we are a trusted name in genetic testing and molecular diagnostics. With 100+ independent intellectual property rights and 200 international certifications, our solutions have made significant contributions to disease prevention and control. For any inquiries or further information, please feel free to contact us directly.</p><p><strong>Reference</strong></p><p>[1] Available at: https://pmc.ncbi.nlm.nih.gov/articles/PMC7265510/</p><p>[2] Available at: https://pubmed.ncbi.nlm.nih.gov/18766252/</p><p>[3] Available at: https://academic.oup.com/eurheartj/article/28/14/1702/2887650</p><p>[4] Available at: https://pmc.ncbi.nlm.nih.gov/articles/PMC10014202/</p><p>[5] Available at: https://pmc.ncbi.nlm.nih.gov/articles/PMC2492913/</p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p></p><p><br/></p>" ["url"] => string(62) "/news/blog/aspirin-resistance-testing-reasons-and-methods.html" ["count"] => string(3) "168" ["sort"] => string(1) "0" ["command"] => string(1) "0" ["isdel"] => string(1) "0" ["free"] => string(1) "0" ["status"] => string(1) "1" ["uid"] => string(1) "4" ["username"] => string(11) "system-2022" ["euid"] => string(1) "0" ["edituser"] => string(0) "" ["input_time"] => string(10) "1730351959" ["create_time"] => string(10) "1730352455" ["update_time"] => NULL ["static_title"] => string(0) "" ["tag"] => NULL ["out_url"] => string(0) "" ["custom_code"] => string(0) "" ["jie"] => string(159) "Aspirin resistance poses a significant challenge for cardiovascular patients. Accurate aspirin resistance testing methods are required for precision medicine. " ["is_ding"] => string(1) "0" ["zname"] => string(0) "" ["index_img"] => array(2) { ["thumb"] => string(52) "/Public/Uploads/uploadfile/images/20241031/3-506.png" ["alt"] => string(9) "3-506.png" } ["vid"] => string(0) "" ["command_detail"] => string(1) "0" ["title_url"] => string(1) "1" ["url_title"] => string(1) "1" ["iframe"] => string(0) "" }
Aspirin resistance poses a significant challenge for cardiovascular patients. Accurate aspirin resistance testing methods are required for precision medicine.
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2024-10-17An Ultimate Solution for Diagnosing Feline Respiratory Distress
array(34) { ["id"] => string(3) "678" ["catid"] => string(3) "498" ["title"] => string(63) "An Ultimate Solution for Diagnosing Feline Respiratory Distress" ["thumb"] => array(2) { ["thumb"] => string(49) "/Public/Uploads/uploadfile/images/20241017/bb.jpg" ["alt"] => string(6) "bb.jpg" } ["keywords"] => string(0) "" ["description"] => string(0) "" ["content"] => string(11025) "<p>Just as is the case with humans, cats may become vulnerable to respiratory diseases within a short time. These illnesses begin gradually to interfere with the breathing ability of a cat, thereby affecting its general well-being. If left without treatment, a cat becomes subjected to severe instances of breathing complications, which makes it hard for the cat to eat or even play. Chronic illness, pain, and even death occur over time. But what if there existed an easy way of early diagnosis of such <a href="https://www.medtl.net/products/feline-respiratory-5-types-pathogen-panel-nucleic-acid-detection-kit-fluorescence-pcr-method.html" target="_self">feline respiratory distress</a>?</p><p style="text-align: center;"> <img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241017/1729149786211430.png" title="" alt="aa.png"/></p><p style="text-align: center;"><em><span style="font-size: 12px;">(Copyright image from: https://www.freepik.com/free-photo/medium-shot-elderly-man-with-cat_37558871.htm#fromView=search&page=1&position=15&uuid=816ee95a-eef6-477e-ac7c-e222b996f8fb)</span></em></p><p><strong>Why Cats Are Susceptible to Respiratory Diseases?</strong></p><p>Cats are prone to feline respiratory distress infections. It often happens this way because cats will find each other at animal shelters, homes, or outdoors. Such diseases are caused by pathogens, like viruses and bacteria. Once the pathology has affected a cat's respiratory system, it normally demonstrates symptoms characterized by sneezing, coughing, nasal discharge, and difficulty breathing. Such clinical signs are elements of the feline respiratory disease complex and can make a cat feel pretty ill and affect its overall rate of respiration. These cats need to be diagnosed in time and get the proper treatment. Therefore, a reliable tool that identifies typical pathogens responsible for such diseases will be helpful.</p><p></p><p><strong>Common Causes of Respiratory Infections in Cats</strong></p><p>There are several pathogens responsible for feline respiratory disease complex symptoms. However, the most common ones are:</p><p><strong>1. Feline Herpesvirus (FHV-1)</strong></p><p>This is one of the critical causes of feline respiratory distress. Its symptoms include sneezing, eye discharge, and nasal congestion. The FHV-1 virus is very contagious since once a cat is infected, it carries it its whole life. Thus, at times of stress, the virus may reactivate and cause new diseases.</p><p><strong>2. Feline Calicivirus (FCV)</strong></p><p>FCV is another contagious virus that affects the respiratory system and can cause oral diseases. It often co-occurs with FHV-1 and is responsible for many upper respiratory infections (URIs) in cats. Symptoms include sneezing, nasal discharge, ulcers in the mouth (especially on the tongue and gums), drooling, fever, and joint pain. Some strains of FCV can cause severe respiratory distress.</p><p><strong>3. Bordetella bronchiseptica (Bb)</strong></p><p>Bordetella bronchiseptica is a bacterial pathogen that commonly causes respiratory infections in dogs but can also infect cats. It’s especially prevalent in high-density environments like shelters and catteries. While it typically causes mild symptoms, it can be more dangerous in kittens, older cats, or those with weakened immune systems.</p><p><strong>4. Mycoplasma Felis (MF)</strong></p><p>Mycoplasma felis is a type of bacteria that lacks a cell wall, making it unique from other bacteria. It can infect the respiratory system, as well as the eyes, causing respiratory infections and conjunctivitis. Mycoplasma infections can be chronic and challenging to treat, particularly when combined with other feline respiratory distress. In some cases, it can lead to pneumonia.</p><p><strong>5. Chlamydophila Felis (CF)</strong></p><p>This bacterium mainly causes conjunctivitis and cat eye infections and can be cat-to-cat transmitted through direct contact. CF infection is mainly observed in cats that share their environments, for instance, those staying in a cattery or a shelter. This bacterium spreads through direct contact with infected cats, mainly through eye secretions. It can also be spread via contaminated objects like bedding or food bowls.</p><p>These five pathogens are the main causes of feline respiratory distress. Early determination of the appropriate pathogen will mean providing the cat with the correct treatment for its condition, improving the cat's health.</p><p><strong>Diagnostic Techniques for Feline Respiratory Distress</strong></p><p>Diagnosis of a feline showing signs of respiratory distress is made based on various methods by which veterinarians identify the cause of distress. These diagnostic techniques commonly include:</p><p><strong>1. Serological Testing</strong></p><p>This is an in-blood testing technique whereby one checks whether there are antibodies or antigens in the blood of a cat associated with particular disease-causing pathogens. Serological testing is often employed when veterinarians suspect viral or bacterial infections and want to confirm a diagnosis based on immune response, particularly when combined with clinical symptoms.</p><p><strong>2. Radiography and Imaging Techniques</strong></p><p>X-rays or other imaging techniques are often available so the veterinarian can see through the body to see the lungs and chest area; changes in form, such as fluid buildup or signs of inflammation, may indicate a respiratory disease. Radiography is commonly used to assess the severity of respiratory distress, particularly when secondary complications like pneumonia, bronchitis, or pleural effusion are suspected.</p><p><strong>3. PCR Testing</strong></p><p>One of the most reliable methods adopted in detecting pathogens is Polymerase Chain Reaction or PCR testing. This technique can indicate the existence of particular genetic material coming from either bacteria or viruses. This would thus give an accurate and rapid diagnosis. This technique has been of great importance in the detection of respiratory infections caused by multiple pathogens.</p><p style="text-align: center;"> <img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241017/1729149877136136.png" title="" alt="bb.png"/></p><p style="text-align: center;"><span style="font-size: 12px;">(Copyright image from: https://www.freepik.com/free-photo/white-fluffy-cat-veterinarian-with-cats-animals-couch_13320879.htm#fromView=search&page=1&position=0&uuid=9b9b21b7-b248-4612-b635-001fbb3548bb)</span></p><p></p><p><a href="https://www.medtl.net/products/feline-respiratory-5-types-pathogen-panel-nucleic-acid-detection-kit-fluorescence-pcr-method.html" target="_self"><strong>TIANLONG's Feline Respiratory 5 Types Pathogen Panel Kit:</strong></a><strong> An Efficient and Effective Diagnostic Solution</strong></p><p></p><p></p><p></p><p></p><p></p><p>For anyone looking for the most accurate and efficient diagnostic tool, a perfect solution can be found in TIANLONG's Feline Respiratory 5 Types Pathogen Panel Nucleic Acid Detection Kit (Fluorescence PCR Method). Here are some reasons why this freeze-dried nucleic acid kit is good for the diagnosis of feline respiratory diseases.</p><p><strong>· Accurate Detection</strong></p><p>With the advanced technology of PCR, this kit goes around detecting DNA or RNA of the five most common respiratory pathogens in cats, including Feline Herpesvirus, Feline Calicivirus, Bordetella bronchiseptica, Mycoplasma Felis, and Chlamydophila Felis. The fluorescence PCR method ensures high sensitivity and can easily detect the minute presence of genetic material of feline respiratory distress pathogen. This degree of accuracy helps veterinarians quickly determine what ails a cat.</p><p><strong>· Internal Control</strong></p><p>The kit comprises an internal control for the purification process and is free from contamination. This will provide confidence among users concerning the accuracy and dependability of the results.</p><p><strong>· Easy and Simple to Use</strong></p><p>TIANLONG's Feline Respiratory 5 Types Pathogen Panel Nucleic Acid Detection Kit is simple to use due to its property of freeze-dried. This reagent only requires reconstitution in water before use, reducing the number of preparation steps and potential for errors compared to traditional liquid PCR reagents. As a result, our Tianlong kit can be highly recommended for veterinary clinics, research labs, and animal shelters for treating feline respiratory distress.</p><p><strong>How Feline Respiratory 5 Types Pathogen Panel Nucleic Acid Detection Kit Works?</strong></p><p>It is quite easy to use TIANLONG's Feline Respiratory 5 Types Pathogen Panel Nucleic Acid Detection Kit. Here are the basic steps as follows:</p><p><strong>· Sample Collection</strong></p><p>Sample collection aims to collect a sample from the cat that may contain genetic material (DNA or RNA) of the pathogens causing the respiratory infection. The collected swabs are then placed in a transport medium to preserve any pathogens present until laboratory processing.</p><p><strong>· Nucleic Acid Extraction</strong></p><p>This step aims to isolate and purify the nucleic acids (DNA or RNA) from the pathogens present in the collected sample. This step is crucial because PCR requires pure nucleic acid to accurately amplify and detect specific pathogen sequences.</p><p><strong>· PCR Detection</strong></p><p>This step aims to detect the presence of specific pathogen genetic material by amplifying targeted sequences using real-time PCR. Fluorescence is used to quantify the amount of nucleic acid during the amplification process. The test results indicate the presence or absence of the five ordinary feline respiratory pathogens based on the fluorescence signal during PCR. If a pathogen’s genetic material is detected, the corresponding pathogen is identified as the cause of the respiratory distress.</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241017/1729149957778328.png" title="" alt="cc.png"/></p><p></p><p></p><p><strong>Conclusion</strong></p><p>Our TIANLONG’s Feline Respiratory 5 Types Pathogen Panel Nucleic Acid Detection Kit has been effective in the diagnosis of feline respiratory distress. As this diagnostic kit is accurate and user-friendly in its design, results acquired through it are reliable. This makes them ideal for veterinary clinics, any research laboratory that wants to lower the feline respiratory rate or even a pet owner who sincerely cares for his/her animals.</p><p>For more details on this product, as well as other diagnostic tools by TIANLONG, visit our entire lineup of diagnostic instruments. </p>" ["url"] => string(79) "/news/blog/an-ultimate-solution-for-diagnosing-feline-respiratory-distress.html" ["count"] => string(3) "175" ["sort"] => string(1) "0" ["command"] => string(1) "0" ["isdel"] => string(1) "0" ["free"] => string(1) "0" ["status"] => string(1) "1" ["uid"] => string(1) "4" ["username"] => string(11) "system-2022" ["euid"] => string(1) "0" ["edituser"] => string(0) "" ["input_time"] => string(10) "1729149716" ["create_time"] => string(10) "1729149992" ["update_time"] => NULL ["static_title"] => string(0) "" ["tag"] => NULL ["out_url"] => string(0) "" ["custom_code"] => string(0) "" ["jie"] => string(148) "Diagnosis of a feline showing signs of respiratory distress is made based on various methods by which veterinarians identify the cause of distress. " ["is_ding"] => string(1) "0" ["zname"] => string(0) "" ["index_img"] => array(2) { ["thumb"] => NULL ["alt"] => NULL } ["vid"] => string(0) "" ["command_detail"] => string(1) "0" ["title_url"] => string(1) "1" ["url_title"] => string(1) "1" ["iframe"] => string(0) "" }
Diagnosis of a feline showing signs of respiratory distress is made based on various methods by which veterinarians identify the cause of distress.
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2024-10-17Food Safety Monitoring for Lab Researchers: Mission, Indicator & Method
array(34) { ["id"] => string(3) "677" ["catid"] => string(3) "498" ["title"] => string(71) "Food Safety Monitoring for Lab Researchers: Mission, Indicator & Method" ["thumb"] => array(2) { ["thumb"] => string(48) "/Public/Uploads/uploadfile/images/20241017/a.jpg" ["alt"] => string(5) "a.jpg" } ["keywords"] => string(0) "" ["description"] => string(0) "" ["content"] => string(8627) "<p>Research labs play a critical role in ensuring food quality so that consumers can have a healthy eating environment. They use microbiological and chemical testing to determine whether the food contains microbes or pathogens. It is their utmost responsibility to maintain a healthy food supply chain by detecting hazards and meeting safety standards. This food safety monitoring helps reduce the risk of foodborne diseases and retrain consumer trust.</p><p>So, in today’s article, we are going to provide a detailed guide on how they carry out effective monitoring for high standards of the food, seeing what mission, indicator, and methods for food safety monitoring in labs.</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241017/1729149398747503.jpg" title="" alt="a.jpg"/></p><p></p><p><strong>The mission of Food Safety Monitoring in Lab</strong></p><p>The primary purpose of lab researchers in food safety monitoring is to ensure that all food products are safe to consume. It involves various detection methods, preventive measures, and expertise to check contamination.</p><p>So, the ultimate mission is to identify the risks before letting them reach the market, assessing the chemical and biological hazards in food during distribution. It includes knowledge of identification, detecting methods, analysis, monitoring, and extracting reports. Along with detecting hazards, labs also help in preventing contamination during food production.</p><p>Besides, they also ensure the whole process follows the set standards of food safety and regulations, like the FDA, EFSA, etc.</p><p>They also check and analyze previous statistics and reports to avoid future hazards and potential risks. The safe food standards are set by authorities.</p><p><strong>Key Indicators of Food Safety Monitoring</strong></p><p>Food safety monitoring or testing commonly examines food chemical composition, the presence of pathogens, physical properties, and the potential for unsafe ingredients. Lab researchers use specific measures to check the contamination or hazards in food. Here are the key indicators that determine how safe the food is for consumption.</p><p></p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241017/1729149431571408.png" title="" alt="b.png"/></p><p><strong>Microorganisms in Food Safety Concerns</strong></p><p>Compared to other contaminations, microorganisms multiply, cause foul odor in the food, and show spoilage signs. Infections occur when you eat the contaminated food, and bacteria continue to multiply in your gastrointestinal system. It could render 128,000 hospitalized, and even 3,000 die every year in the United States. Patients always have the following symptoms:</p><ul style="list-style-type: disc;"><li><p>Fever</p></li><li><p>Nausea or Vomiting</p></li><li><p>Diarrhea & Stomach Cramping</p></li></ul><p>As a result, undergoing food safety monitoring aims to find out the cause of foodborne illness. More importantly, it is a kind of social responsibility to ensure public health. </p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241017/1729149468666771.png" title="" alt="c.png"/></p><p></p><p><strong>Monitoring Tools for Microbicidal Indicators of Food Safety</strong></p><p>For food safety monitoring, there are tools that lab researchers use to detect and test microbicidal indicators. These help them have an estimation of the microbes and ATP count in the food. Here are the tools and their usage in food monitoring:</p><p><strong>1. Microscopy</strong></p><p>Food contamination is detected with various analysis methods because of safety reasons. For this analysis, the lab researchers use microscopy, a device to identify microorganisms in prepared food. It allows a direct look at bacteria, yeast, molds, and other contamination.</p><p>Different microscopes are used to identify different microbial contamination that are as follows:</p><p>· Stereo Microscopes: Used for spotting foreign materials in food at low magnification.</p><p>· Polarizing Microscope: Identifies microbes in fatty or fibrous foods.</p><p>· Fluorescence Microscope: Detects bacteria like Staphylococcus aureus and E. coli using gram staining.</p><p>· Scanning Electron Microscopes: Essential for examining high-fat or high-water foods and controlling moisture and pressure.</p><p><strong>2. Total Plate Count (TPC) and Aerobic Plate Count (APC)</strong></p><p>TPC is a method used to determine the total number of bacteria in a food sample, while APC is used as an indicator to tell the populations of bacteria in food samples. These serve as excellent indicators in food safety monitoring to determine the contamination and effectiveness of particles. Lab researchers use them for various stages in food production, from ingredients to the final product.</p><p><strong>3. ATP Monitoring System</strong></p><p>The ATP monitoring system is a device used to meet the ATP test standards of the given food sample. Because of the presence of ATP, these foods are more likely to be contaminated by microorganisms. When food is combined with chemicals in the sampler, a chemiluminescent reaction occurs that results in light. The amount of light decides how much ATP is present in the food.</p><p><strong>Introducing Tianlong’s Biolum Portable ATP Monitoring System Pro</strong></p><p>Our<a href="https://www.medtl.net/products/biolum-portable-atp-monitoring-system-pro.html" target="_self"> Tianlong’s Biolum Portable ATP Monitoring System Pro</a> is a powerful and convenient ATP testing solution that can deliver accurate food safety monitoring results in labs. The key features of Biolum Portable ATP Monitoring System Pro are as follows:</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241017/1729149550497805.png" title="" alt="d.png"/></p><p></p><ul style="list-style-type: disc;"><li><p><strong>Exceptional Sensitivity</strong></p></li></ul><p>Capable of detecting as little as 10-16 mol of ATP, the Biolum Portable ATP Monitoring System Pro offers unparalleled accuracy in assessing surface cleanliness, ensuring the highest standards of hygiene.</p><ul style="list-style-type: disc;"><li><p><strong>Rapid Verification</strong></p></li></ul><p>The Biolum Portable ATP Monitoring System Pro delivers swift sanitation verification, with each test completed in just 10 seconds. This rapid testing capability ensures immediate feedback and efficient workflow in any setting.</p><ul style="list-style-type: disc;"><li><p><strong>Pre-configured Templates</strong></p></li></ul><p>To accommodate the diverse needs of different sectors and locations, the Biolum Portable ATP Monitoring System Pro comes equipped with an array of pre-configured templates. This feature simplifies the setup process and then undergoes immediate deployment across a variety of environments.</p><ul style="list-style-type: disc;"><li><p><strong>Compatible Lyophilized Surface Swab</strong></p></li></ul><p>Our ATP testing system is designed to work seamlessly with the ATP Lyophilized Surface Swab—a user-friendly and effective tool for collecting samples. This compatibility ensures that users can conduct thorough and reliable tests with minimal training.</p><ul style="list-style-type: disc;"><li><p><strong>Cloud-Based Data Management</strong></p></li></ul><p>With its sophisticated cloud-based platform, Tianlong’s Biolum Portable ATP Monitoring System Pro enables comprehensive data management. Users can effortlessly monitor, track, and analyze testing results from multiple facilities, enhancing the ability to manage risks and maintain compliance.</p><p></p><p><strong>Conclusion</strong></p><p>Food safety monitoring is essential for lab researchers. With the help of different tools and systems, they can fetch results for different food cleanliness purposes. Using the right tool enables researchers to have quicker and more accurate results, which makes handling various tests easy.</p><p>At Tianlong, we are an innovative R&D company established in 1997 to provide testing instruments, products, and solutions. We have delivered our products in 100+ countries and regions and have 80+ NMPA approvals and 200 international certificates. As a result, you can trust our ATP testing device. If you are interested, please visit our Tianlong’s official website to learn more and contact us!</p>" ["url"] => string(87) "/news/blog/food-safety-monitoring-for-lab-researchers-mission-indicator-amp-method.html" ["count"] => string(3) "116" ["sort"] => string(1) "0" ["command"] => string(1) "0" ["isdel"] => string(1) "0" ["free"] => string(1) "0" ["status"] => string(1) "1" ["uid"] => string(1) "4" ["username"] => string(11) "system-2022" ["euid"] => string(1) "0" ["edituser"] => string(0) "" ["input_time"] => string(10) "1729149345" ["create_time"] => string(10) "1729149712" ["update_time"] => NULL ["static_title"] => string(0) "" ["tag"] => NULL ["out_url"] => string(0) "" ["custom_code"] => string(0) "" ["jie"] => string(137) "In today’s article, we are going to provide a detailed guide on how they carry out effective monitoring for high standards of the food." ["is_ding"] => string(1) "0" ["zname"] => string(0) "" ["index_img"] => array(2) { ["thumb"] => NULL ["alt"] => NULL } ["vid"] => string(0) "" ["command_detail"] => string(1) "0" ["title_url"] => string(1) "1" ["url_title"] => string(1) "1" ["iframe"] => string(0) "" }
In today’s article, we are going to provide a detailed guide on how they carry out effective monitoring for high standards of the food.
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2024-10-11ATP Monitoring: Why Is It Important for Food Safety Lab?
array(34) { ["id"] => string(3) "674" ["catid"] => string(3) "498" ["title"] => string(56) "ATP Monitoring: Why Is It Important for Food Safety Lab?" ["thumb"] => array(2) { ["thumb"] => string(60) "/Public/Uploads/uploadfile/images/20241011/3281728617697.png" ["alt"] => string(0) "" } ["keywords"] => string(0) "" ["description"] => string(0) "" ["content"] => string(8961) "<p>Ensuring food safety should be the utmost priority of every food industry professional. A single lapse in hygiene can lead to significant health issues. According to a report by WHO, almost 420,000 people die every year by eating contaminated food<sup>[1]</sup>.</p><p>ATP Labs guarantee new cleanliness standards by testing germs and organic residues on surfaces. In a food safety lab, ATP testing gadgets are used as critical markers for unhygienic surfaces.</p><p>Below, we have shared how <a href="https://www.medtl.net/products/biolum-portable-atp-monitoring-system-pro.html" target="_self">ATP monitoring</a> devices have revolutionized cleanliness standards in food safety labs.</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241011/1728617763388982.png" title="" alt="1.png"/></p><p> </p><p><strong>ATP, Explained</strong></p><p>ATP (Adenosine Triphosphate) is an organic molecule in the cell membranes of living organisms. Often termed the "energy source" of cells, it is a nucleotide helping the human body in various functions. Mostly, it is used during muscle contraction, nerve impulse propagation, and chemical synthesis. An enzyme ATP synthase produces ATP in cells<sup>[2]</sup>.</p><p>The Adenosine Triphosphate test is used for preventing viral infections in the healthcare and food sectors. Since ATP is found in every living organism, its presence indicates if there is any biological material present on a surface. In a food safety lab, it is being tested using a detector called a Luminometer.</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241011/1728617773664394.png" title="" alt="2.png"/></p><p style="text-align: center;"><em><span style="font-size: 12px;">Public domain image from: https://en.wikipedia.org/wiki/Adenosine_triphosphate</span></em></p><p><strong>Pathogenic Bacteria Rendering Food Safety</strong></p><p>The main reason for spoiled or unhygienic food is the presence of pathogens in it. As time passes, bacteria, fungi, and other microorganisms multiply on the surface of food, making it unsafe for consumption.</p><p>Due to certain environmental conditions, pH, and temperature levels, these pathogens continue to grow. For instance, bacteria grow in huge numbers at 37 °C, which is the room temperature in many regions. That’s why it is the first microbe to be grown on food.</p><p>Here are some of the most common foodborne pathogens:</p><p><strong>1. Salmonella</strong></p><ul style="list-style-type: disc;"><li><p><strong>Potential Period:</strong> Patients get symptoms within 8 to 72 hours after exposure<sup>[3]</sup>.</p></li><li><p><strong>Potential Risks:</strong> Salmonella causes salmonellosis or salmonella infection, leading to upset stomach, diarrhea, fever, and pain.</p></li><li><p><strong>Infectious Dose: </strong>103 bacilli (non-typhoidal) and 105 bacilli (enteric)<sup>[4]</sup>.</p></li></ul><p><strong>2. Listeria Monocytogenes</strong></p><ul style="list-style-type: disc;"><li><p><strong>Potential Period: </strong>Symptoms appear after a few hours, or it may take 2-3 days.</p></li><li><p><strong>Potential Risks: </strong>Causes listeriosis, leading to muscle aches, nausea, stiffness in the neck, headaches, and stomach pain.</p></li><li><p><strong>Infectious Dose:</strong> 10 to 100 million CFU(healthy hosts), and 0.1 to 10 million CFU( individuals at high risk of infection)<sup>[5]</sup>.</p></li></ul><p><strong>3. Vibrio Parahaemolyticus</strong></p><ul style="list-style-type: disc;"><li><p><strong>Potential Period:</strong> Symptoms typically appear 6 hours to 4 days after ingestion<sup>[6]</sup>.</p></li><li><p><strong>Potential Risks:</strong> It is usually caused by eating contaminated seafood. The disease causes watery diarrhea, often with abdominal cramping, nausea, vomiting, fever, and chills.</p></li><li><p><strong>Infectious Dose:</strong> 107 to 108 organisms<sup>[7]</sup></p></li></ul><p><strong>4. Bacillus Cereus</strong></p><ul style="list-style-type: disc;"><li><p><strong>Potential Period:</strong> It usually takes 1-6 hours after ingestion to show symptoms<sup>[8]</sup>.</p></li><li><p><strong>Potential Risks:</strong> Causes nausea, vomiting, abdominal cramps and diarrhea.</p></li><li><p><strong>Infectious Dose:</strong> 104-109 cells per gram of food(diarrheal type) and105-108 cells per gram of food(emetic type)<sup> [9]</sup>.</p></li></ul><p><strong>5. Clostridium Botulinum</strong></p><ul style="list-style-type: disc;"><li><p><strong>Potential Period:</strong> Symptoms generally appear 12-36 hours after exposure.</p></li><li><p><strong>Potential Risks: </strong>Causes botulism, a rare but serious illness that can lead to marked fatigue, weakness, and vertigo, usually followed by blurred vision, dry mouth, and difficulty in swallowing and speaking. Sometimes, it may lead to respiratory failure and paralysis.</p></li><li><p><strong>Infectious Dose: </strong>Estimated lethal dose is 1.3-2.1 ng/kg in humans<sup>[10]</sup>.</p></li></ul><p><br/></p><p><strong>Significance of ATP Monitoring for Microorganism Detection in Food Safety Lab</strong></p><p>ATP monitoring is the ultimate test that is used for microorganism detection in a food safety lab. It is extremely useful to ensure food safety and optimum health conditions. By detecting the presence of ATP on surfaces, food safety professionals can quickly identify areas that may be contaminated with organic matter or microbial growth. And unlike traditional microbiological testing, ATP monitoring provides real-time results, enabling rapid decision-making and proactive measures to maintain a hygienic environment.</p><p><br/></p><p><strong>Tianlong's Biolum Portable ATP Monitoring System Pro</strong></p><p style="text-align: center;"><strong><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20241017/1729148620880241.jpg" title="" alt="3.jpg"/></strong></p><p>Our Tianlong’s Biolum Portable ATP Monitoring System Pro is a robust tool designed for accurate hygiene monitoring in a food safety lab.</p><p>It provides rapid sanitation verification within 10 seconds of testing. You can easily use the device and can detect 10-16 mol of ATP present on a surface. With a 5-inch shatter-proof touchscreen, easy-to-use UI, and USB-C port for convenient charging, Biolum Pro has made its mark in food safety insurance. </p><p><br/></p><p><strong>Conclusion</strong></p><p>Tianlong is an R&D company that introduced the Biolum Portable ATP Monitoring System, which provides rapid and accurate results. We have been accredited with certifications from the prestigious AOAC Research Institute. Choose Tialong for high-quality and efficient instruments to ensure hygiene in the food safety lab!</p><p>References</p><p>[1] Food safety. Available at: https://www.who.int/news-room/fact-sheets/detail/food-safety (Accessed: 2th, August)</p><p>[2] Respiratory Chain and ATP Synthase. Available at: https://www.sciencedirect.com/topics/neuroscience/atp-synthase (Accessed: 2th, August)</p><p>[3] Salmonella infection. Available at: https://www.mayoclinic.org/diseases-conditions/salmonella/symptoms-causes/syc-20355329 (Accessed: 2th, August)</p><p>[4] Pathogen Safety Data Sheets: Infectious Substances – Salmonella enterica spp. Available at: https://www.canada.ca/en/public-health/services/laboratory-biosafety-biosecurity/pathogen-safety-data-sheets-risk-assessment/salmonella-enterica.html (Accessed: 2th, August)</p><p>[5] Listeria monocytogenes: Infectious substances Pathogen Safety Data Sheet. Available at: https://www.canada.ca/en/public-health/services/laboratory-biosafety-biosecurity/pathogen-safety-data-sheets-risk-assessment/listeria-monocytogenes.html (Accessed: 2th, August)</p><p>[6] Vibrio parahaemolyticus. Available at: https://www.sciencedirect.com/topics/medicine-and-dentistry/vibrio-parahaemolyticus (Accessed: 2th, August)</p><p>[7] Vibrio Parahaemolyticus. Available at: https://www.ehs.com/resources/sds-resources/free-safety-data-sheet-index/vibrio-parahaemolyticus/ (Accessed: 2th, August)</p><p>[8] Preventing Foodborne Illness: Bacillus cereus. Available at: https://www.nifa.usda.gov/sites/default/files/resource/Preventing-Foodborne-Illness-Bacillus-cereus.pdf (Accessed: 2th, August)</p><p>[9] Bacillus cereus, excluding biovar anthracis: Infectious substances pathogen safety data sheet. Available at: https://www.canada.ca/en/public-health/services/laboratory-biosafety-biosecurity/pathogen-safety-data-sheets-risk-assessment/bacillus-cereus.html (Accessed: 2th, August)</p><p>[10] Clostridium botulinum. Available at: https://en.wikipedia.org/wiki/Clostridium_botulinum (Accessed: 2th, August)</p>" ["url"] => string(70) "/news/blog/atp-monitoring-why-is-it-important-for-food-safety-lab.html" ["count"] => string(3) "158" ["sort"] => string(1) "0" ["command"] => string(1) "0" ["isdel"] => string(1) "0" ["free"] => string(1) "0" ["status"] => string(1) "1" ["uid"] => string(1) "4" ["username"] => string(11) "system-2022" ["euid"] => string(1) "4" ["edituser"] => string(11) "system-2022" ["input_time"] => string(10) "1728617497" ["create_time"] => string(10) "1728617697" ["update_time"] => string(10) "1729148640" ["static_title"] => string(0) "" ["tag"] => NULL ["out_url"] => string(0) "" ["custom_code"] => string(0) "" ["jie"] => string(95) "In a food safety lab, ATP testing gadgets are used as critical markers for unhygienic surfaces." ["is_ding"] => string(1) "0" ["zname"] => string(0) "" ["index_img"] => array(2) { ["thumb"] => NULL ["alt"] => NULL } ["vid"] => string(0) "" ["command_detail"] => string(1) "0" ["title_url"] => string(1) "1" ["url_title"] => string(1) "1" ["iframe"] => string(0) "" }
In a food safety lab, ATP testing gadgets are used as critical markers for unhygienic surfaces.
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2024-09-29Tianlong's ATP Hygiene Monitoring System: Advancing Food Safety through Rigorous Testing
array(34) { ["id"] => string(3) "663" ["catid"] => string(3) "498" ["title"] => string(88) "Tianlong's ATP Hygiene Monitoring System: Advancing Food Safety through Rigorous Testing" ["thumb"] => array(2) { ["thumb"] => string(50) "/Public/Uploads/uploadfile/images/20240929/qqq.jpg" ["alt"] => string(7) "qqq.jpg" } ["keywords"] => string(0) "" ["description"] => string(0) "" ["content"] => string(5457) "<p>Tianlong is proud to announce the successful validation of its <a href="https://www.medtl.net/products/biolum-portable-atp-monitoring-system-pro.html" target="_self">Biolum Portable ATP Hygiene Monitoring System</a>, which has been certified by the Association of Official Analytical Chemists (AOAC) Research Institute in 2024 (Certificate No. 022401). This innovative system utilizes cutting-edge bioluminescence technology to provide rapid and reliable detection of adenosine triphosphate (ATP), a key indicator of cleanliness in food safety management.</p><p><br/></p><p><strong>Background on ATP Detection</strong></p><p style="text-align: center;"><strong><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20240929/1727581064756653.png" title="" alt="1.png" width="459" height="343" style="width: 459px; height: auto;"/></strong></p><p>ATP is a ubiquitous organic compound found in all biological materials, including microorganisms and food residues. The measurement of ATP on surfaces can be effectively conducted through luciferase-based luminometer assays, which produce relative light units (RLUs). Low RLU readings indicate effective cleaning, while high readings necessitate further cleaning efforts to ensure hygiene standards are met.</p><p><strong><br/></strong></p><p><strong>Tianlong’s ATP Hygiene Monitoring System</strong></p><p>The Biolum Portable ATP Hygiene Monitoring System produced by Tianlong provides a bioluminescence assay for monitoring hygiene and cleanliness standards when used with ATP Test Swabs (e.g., ATP QuickSwab). Our ATP swabs are pen-sized sampling device that contain recombinant luciferase reagent for detecting the amount of ATP on the basis of admitted fluorescence and to generate visualized results in the form of relative light units (RLUs).</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20240929/1727581794315740.png" title="" alt="aaaaa.png"/></p><p>Combined with the testing device, Tianlong Biolum Portable ATP Hygiene Monitoring System is sensitive enough to detect ATP in concentration as low as 10-16 mol within 10 seconds. Moreover, it shows good linearity and sensitivity both in pure analyte studies and ATP from and in presence of food and microbial spoilage.</p><p> </p><p><strong>Validation from Independent Laboratory Studies</strong></p><p>To objectively validate the performance of the Tianlong system, independent laboratory studies were conducted.</p><ul style="list-style-type: disc;"><li><p>Methods of validation</p></li></ul><p>An independent laboratory study was conducted with evaluation of two matrices for stainless steel surface: sweetened yogurt residue and doughnut residue. Prior to application of the matrix, the stainless steel was sanitized by using a UV lamp for 20 minutes, and then cleaned with a 10% Contrad 70 cleaning solution in water followed by a rinse with sterile water. The surface was then washed with 70% isopropanol and allowed to air dry.</p><p>For each matrix, 10 replicates were evaluated at the following levels: <10, 10-30, 30-100, 100-300, and 300-1000 RLUs. Each contamination level was homogenized by stomaching for 2 minutes and then spread over a 4”x 4”stainless steel surface area. For the blank level (<10 RLU), an aliquot of sterile water was spread over a 4”x 4”surface area. Regions to be measured allowed to dry for 5 minutes after surface contamination for the wet sampling evaluation, and 1 hour after surface contamination for the dry sampling evaluation. The RLUs were calculated and reported for each level for both sampling times for both matrices.</p><ul style="list-style-type: disc;"><li><p>Results Demonstrate Consistency and Accuracy</p></li></ul><p>For the matrix study, all replicates evaluated were within the target RLU levels for both matrices (i.e., sweetened yogurt residue and doughnut residue) and for both wet and dry sampling (Figure 1 & 2).</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20240929/1727581336484522.png" title="" alt="ddd.png"/></p><p>For each targeted RLU levels, the box and whiskers in the box-plot for each matrix showed compact distribution, indicating highly consistent results across tests, which was greatly benefit from the testing accuracy and stability of our products. It is also worth noting that the detection performance of our products on dry samples was as good as that on wet samples, enabling their flexible application in a variety of production practice scenarios without performance degradation.</p><p> </p><p><strong>Conclusion</strong></p><p>The AOAC certification highlights the exceptional quality and reliability of Tianlong's Biolum Portable ATP Hygiene Monitoring System. By providing an accurate and efficient method for ATP detection, Tianlong is committed to supporting industries in maintaining high hygiene standards and ensuring food safety.</p><p><br/></p><p>For further information on the performance validation and detailed results, please visit the <a href="https://members.aoac.org/AOAC/PTM_Validated_Methods.aspx" target="_self">AOAC</a> website or contact us directly.</p><p><strong>Contact:</strong> </p><p>Tianlong Science and Technology</p><p>Mail: inquiry@medtl.com</p><p>Phone: +86-29-82682132</p><p>Address: No. 4266 Shanglin Road, Xi'an, China</p>" ["url"] => string(102) "/news/blog/tianlongs-atp-hygiene-monitoring-system-advancing-food-safety-through-rigorous-testing.html" ["count"] => string(3) "376" ["sort"] => string(1) "0" ["command"] => string(1) "1" ["isdel"] => string(1) "0" ["free"] => string(1) "0" ["status"] => string(1) "1" ["uid"] => string(1) "4" ["username"] => string(11) "system-2022" ["euid"] => string(1) "4" ["edituser"] => string(11) "system-2022" ["input_time"] => string(10) "1727581005" ["create_time"] => string(10) "1727581515" ["update_time"] => string(10) "1728711417" ["static_title"] => string(0) "" ["tag"] => NULL ["out_url"] => string(0) "" ["custom_code"] => string(0) "" ["jie"] => string(175) "By providing an accurate and efficient method for ATP detection, Tianlong is committed to supporting industries in maintaining high hygiene standards and ensuring food safety." ["is_ding"] => string(1) "0" ["zname"] => string(0) "" ["index_img"] => array(2) { ["thumb"] => string(54) "/Public/Uploads/uploadfile/images/20240929/qqq-659.jpg" ["alt"] => string(11) "qqq-659.jpg" } ["vid"] => string(0) "" ["command_detail"] => string(1) "0" ["title_url"] => string(1) "1" ["url_title"] => string(1) "1" ["iframe"] => string(0) "" }
By providing an accurate and efficient method for ATP detection, Tianlong is committed to supporting industries in maintaining high hygiene standards and ensuring food safety.
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2024-07-16Urine PCR Test: How Effective Is It in Detecting Urinary Tract Infection?
array(34) { ["id"] => string(3) "622" ["catid"] => string(3) "498" ["title"] => string(73) "Urine PCR Test: How Effective Is It in Detecting Urinary Tract Infection?" ["thumb"] => array(2) { ["thumb"] => string(50) "/Public/Uploads/uploadfile/images/20240716/aaa.png" ["alt"] => string(7) "aaa.png" } ["keywords"] => string(0) "" ["description"] => string(0) "" ["content"] => string(11548) "<p>Urinary Tract Infections (UTIs) are common urinary diseases affecting people across the globe. They occur when the bacteria infect the urinary tract, including the kidneys, ureters, bladder, and urethra. Reports indicate that nearly 60% of women and 12% of men worldwide[1] experience this condition at least once in their lifetime. This translates into hundreds of millions of people needing proper diagnosis and treatment for UTIs.<br/></p><p>So, in today’s article, we are going to explore the current approaches to detecting UTIs, focusing on what a <a href="https://www.medtl.net/products/instruments/real-time-pcr-systems" target="_self">urine PCR test </a>is and how it can deliver an effective and accurate result.</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20240716/1721097813623828.png" title="" alt="1.png"/></p><p></p><p><strong>Current Approaches to Diagnosing UTIs</strong></p><p>Like other medical conditions, UTIs also present with a whole set of symptoms that need prompt attention. Common symptoms include frequent urination, a burning sensation during urination, cloudy or strong-smelling urine, and lower abdominal pain. However, these symptoms alone are not sufficient for a definitive diagnosis.</p><p>Modern medical science employs several standard methods for diagnosing UTIs, including urinalysis, urine culture, dipstick test, and urine PCR testing:</p><p><strong>Urinalysis</strong></p><p>This fundamental diagnostic tool involves analyzing a urine sample via microscopy to identify specific chemical compounds, such as leukocyte esterase and nitrites, which indicate a possible UTI.</p><ul style="list-style-type: disc;"><li><p>Urine Culture</p></li></ul><p>This method is recognized as the definitive for identifying the specific bacteria causing UTIs. In this procedure, bacteria from the urine sample are cultured in a controlled laboratory setting, enabling precise identification of the bacterial strain causing the infection.</p><ul style="list-style-type: disc;"><li><p>Dipstick Test</p></li></ul><p>The dipstick test is a rapid method for diagnosing urinary infections. This primarily self-administered approach involves dipping a chemically treated strip into a urine sample, to detect specific chemicals indicative of a UTI.</p><ul style="list-style-type: disc;"><li><p>Urine PCR Testing</p></li></ul><p>The urine Polymerase Chain Reaction (PCR) test represents a cutting-edge advancement in medical diagnostics, particularly in the detection and management of UTIs. Utilizing molecular technology, this test amplifies minuscule amounts of bacterial DNA in urine samples, enabling clinicians to confirm infections and pinpoint the specific pathogens causing a UTI.</p><ul style="list-style-type: disc;"><li><p>Other UTIs Detection Solutions</p></li></ul><p>Sometimes, when the situation worsens, and UTIs occur frequently, physicians may also perform ultrasounds, CT scans, and MRIs to detect structural abnormalities or blockages in the urinary tract.</p><p><strong>Urine PCR Testing vs. Other UTI Detection Solutions</strong></p><p>As mentioned above, urinalysis, urine culture, dipstick test, and urine PCR testing are commonly used in hospitals and labs to diagnose UTIs. Due to its advanced technology, urine PCR testing stands out as an effective measure among various UTI detection techniques. Talking specifically, here’s how:</p><p></p><ul style="list-style-type: disc;"><li><p>1. Urine PCR Testing vs. Urine Culture</p></li></ul><p>The primary distinction between the two diagnostic techniques lies in their time efficiency. Urine culture methods generally require 24 to 48 hours to yield results, whereas urine PCR tests can provide findings within hours.</p><p>Additionally, urine culture methodologies have limitations, they may fail to detect certain bacterial strains that are difficult to culture, resulting in potential false negatives. In contrast, urine PCR tests can detect exceedingly low levels of bacterial DNA, thereby enhancing their sensitivity and reliability, even when bacterial presence is minimal.</p><ul style="list-style-type: disc;"><li><p>2. Urine PCR Testing vs. Urine Gram Stain</p></li></ul><p>Urine Gram stain, a urinalysis approach, involves microscopic examination of a stained urine sample to identify and classify bacteria based on their Gram stain characteristics. It provides information about the presence and staining pattern of bacteria, aiding in early treatment decisions when a UTI is suspected[2]. However, urine Gram stain may be affected by contamination from normal urogenital flora, potentially limiting its accuracy in UTI detection[2].</p><p>Polymerase Chain Reaction (PCR) is a molecular diagnostic technique that amplifies and detects specific DNA sequences in a sample. Urine PCR testing for UTIs detects bacterial or yeast DNA targets in the urine sample with high sensitivity, capable of identifying microorganisms that may be undetected by urine Gram stain[2].</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20240716/1721097838180097.png" title="" alt="2.png"/></p><p></p><ul style="list-style-type: disc;"><li><p>3. Urine PCR Testing vs. Antibiotic Sensitivity Testing</p></li></ul><p>Antibiotic Sensitivity Testing in UTI detection involves determining the susceptibility of urinary pathogens to various antibiotics, guiding effective treatment choices. It typically takes 2-3 days to obtain results[3], potentially delaying appropriate antibiotic treatment. Besides, it only assesses susceptibility to specific antibiotics for the isolated pathogen, lacking information about other pathogens or the overall microbial profile of the infection[4].</p><p>In contrast, urine PCR testing provides results within hours[3], allowing for prompt initiation of appropriate antibiotic treatment. Furthermore, urine PCR testing detects a wide range of pathogens[4], including fastidious organisms. And it can identify multiple pathogens in a single sample to cater to huge detecting demands in hospitals.</p><p><strong>Tianlong’s Gentier X3 Series Real-Time PCR System</strong></p><p>For achieving precision and efficiency in PCR testing, the Tianlong <a href="https://www.medtl.net/products/gentier-x3-series-real-time-pcr-system.html" target="_self">Gentier X3 Series Real-Time PCR System</a> offers an exceptional solution for conducting urine PCR tests. Here’s why:</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20240716/1721097856801451.png" title="" alt="3.png"/></p><ul style="list-style-type: disc;"><li><p>1. Multi-Block Design</p></li></ul><p>Our Gentier X3 Series Real-Time PCR System stands at the forefront of PCR technology with its innovative 3-block design, setting a new standard for versatility in laboratory settings. In this urine PCR test machine, each block operates independently and is highly efficient. Equipped with either 6 or 4 fluorescence channels (optional), each block can perform fluorescence scanning of 32 wells in just 2 seconds. Such rapid data acquisition significantly enhances PCR testing efficiency, particularly in environments with high throughput demands. Besides, each block supports different protocols, allowing for simultaneous testing of multiple samples such as urine, blood, saliva, and more. </p><p>This capability of swift scanning and independent operation ensures that the Gentier X3 Series Real-Time PCR System meets the rigorous demands of busy laboratories, facilitating faster decision-making and significantly improving workflow efficiency.</p><ul style="list-style-type: disc;"><li><p>2. Accurate Temperature Control</p></li></ul><p>Accurate temperature control is critical in urine PCR testing, as it directly impacts DNA replication fidelity and overall test result integrity. Our Gentier X3 Series Real-Time PCR System offers superior temperature precision (≤ 0.1℃), enhanced by a compensation heating function that ensures stable and accurate enzymatic reactions. Furthermore, its advanced hot lid with pressure sensing to prevent consumable deformation and evaporation, thereby preserving sample integrity and enhancing the reliability of diagnostic procedures. These integrated capabilities position the Gentier X3 Series Real-Time PCR System as an indispensable tool for meeting the stringent standards of modern medical diagnostics.</p><ul style="list-style-type: disc;"><li><p>3. Comprehensive Analysis</p></li></ul><p>Tianlong's Gentier X3 Series Real-Time PCR System is equipped with advanced software analysis capabilities that significantly enhance diagnostic precision. This sophisticated system is designed to support a diverse array of analytical functions crucial for accurate urine pathogen detection. These functions include absolute quantification analysis, relative quantification analysis, melting curve analysis, high-resolution melting (HRM), genotyping, and endpoint fluorescence. Together, these capabilities enable Tianlong’s Gentier X3 Series Real-Time PCR System to deliver reliable and comprehensive results crucial for the effective diagnosis and management of UTIs.</p><p></p><p><strong>Conclusion</strong></p><p style="text-align: center;"><strong><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20240716/1721097876530305.png" title="" alt="4.png"/></strong></p><p>In modern medical science, there are various methods for detecting UTIs, among which urine PCR testing stands out for its speed, effectiveness and reliability, making it a commonly used tool in clinical and research settings. Our Tianlong’s Gentier X3 Series Real-Time PCR System represents a highly effective option in this regard. We, <a href="https://www.medtl.net/" target="_self">Tianlong</a>, are a leader in genetic testing and molecular diagnostics, specializing in the development and production of nucleic acid testing instruments such as PCR thermal cyclers and real-time PCR systems. With over 80 NMPA approvals and more than 120 international certifications, our instruments are trusted and used in over 100 countries. They have played crucial roles during health crises like COVID-19, avian influenza, and Ebola, underscoring our commitment to reliability and our significant contribution to disease prevention and control.</p><p>If you are interested, please visit Tianlong’s official website to learn more and contact us!</p><p>Reference</p><p>[1] Available at: https://www.urologyhealth.org/urology-a-z/u/urinary-tract-infections-in-adults</p><p>[2] Available at: https://clpmag.com/diagnostic-technologies/urinalysis-toxicology/urine-analyzers/diagnosing-utis-with-urine-pcr/</p><p>[3] Available at: https://www.degruyter.com/document/doi/10.1515/labmed-2021-0085/html?lang=en</p><p>[4] Available at: https://www.mdpi.com/2079-7737/13/4/257</p>" ["url"] => string(87) "/news/blog/urine-pcr-test-how-effective-is-it-in-detecting-urinary-tract-infection.html" ["count"] => string(3) "297" ["sort"] => string(1) "0" ["command"] => string(1) "0" ["isdel"] => string(1) "0" ["free"] => string(1) "0" ["status"] => string(1) "1" ["uid"] => string(1) "4" ["username"] => string(11) "system-2022" ["euid"] => string(1) "0" ["edituser"] => string(0) "" ["input_time"] => string(10) "1721097239" ["create_time"] => string(10) "1721098002" ["update_time"] => NULL ["static_title"] => string(0) "" ["tag"] => NULL ["out_url"] => string(0) "" ["custom_code"] => string(0) "" ["jie"] => string(181) "In today’s article, we are going to explore the current approaches to detecting UTIs, focusing on what a urine PCR test is and how it can deliver an effective and accurate result." ["is_ding"] => string(1) "0" ["zname"] => string(0) "" ["index_img"] => array(2) { ["thumb"] => NULL ["alt"] => NULL } ["vid"] => string(0) "" ["command_detail"] => string(1) "0" ["title_url"] => string(1) "1" ["url_title"] => string(1) "1" ["iframe"] => string(0) "" }
In today’s article, we are going to explore the current approaches to detecting UTIs, focusing on what a urine PCR test is and how it can deliver an effective and accurate result.
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2024-06-19How to Reduce Chances of Lab Contamination During DNA and RNA Extraction
array(34) { ["id"] => string(3) "617" ["catid"] => string(3) "498" ["title"] => string(72) "How to Reduce Chances of Lab Contamination During DNA and RNA Extraction" ["thumb"] => array(2) { ["thumb"] => string(48) "/Public/Uploads/uploadfile/images/20240619/2.jpg" ["alt"] => string(5) "2.jpg" } ["keywords"] => string(0) "" ["description"] => string(0) "" ["content"] => string(11472) "<p>In pursuing molecular studies, researchers frequently encounter the formidable challenge of lab contamination, particularly during <a href="https://www.medtl.net/products/instruments/nucleic-acid-extractors" target="_self">DNA and RNA extraction</a> processes. Such contamination not only compromises the integrity of scientific data but can lead to significant delays and increased costs in research.</p><p>As scientists seek to unlock the secrets held within genetic materials, extraneous nucleic acids can severely skew results, leading to misleading conclusions. Given the critical importance of accurate data in molecular biology, addressing lab contamination is paramount.</p><p>This article delves into practical strategies to mitigate contamination risks during the crucial stages of DNA and RNA extraction, ensuring more reliable and accurate research outcomes.</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20240619/1718780211312033.png" title="" alt="1.png" width="807" height="533" style="width: 807px; height: auto;"/></p><p><strong>DNA and RNA Extraction Processes: Manual and Automated Methods</strong></p><ul style="list-style-type: disc;"><li><p>Manual Steps</p></li></ul><p>The process of manually extracting DNA and RNA begins with cell lysis, where cell membranes are disrupted to release nucleic acids. This is often achieved through physical means like grinding or using chemical lysing agents. Subsequent steps involve treating the solution to remove proteins and other contaminants, typically using enzymes such as protease or organic solvents.</p><p>After purification, nucleic acids are precipitated from the solution, usually with alcohol, and collected by centrifugation. The final step is washing and resuspending the DNA or RNA in a suitable buffer, preparing it for further analysis.</p><ul style="list-style-type: disc;"><li><p>Automatic Solution: Automated Nucleic Acid Extraction Systems</p></li></ul><p>Automated nucleic acid extraction systems provide a more streamlined and standardized approach compared to manual methods. These systems utilize robotic mechanisms and pre-packed cartridges containing all necessary reagents, processing multiple samples simultaneously.</p><p>The automated process follows the core steps of lysis, purification, and recovery, integrated into a single, closed system. This not only improves efficiency but also minimizes the risk of contamination and human error. Automated systems are particularly valuable in high-throughput settings, enhancing both reproducibility and scalability.</p><p><strong>Risks of Lab Contamination During DNA and RNA Extraction</strong></p><p>Contamination during the extraction of DNA and RNA can occur at various stages, primarily due to the sensitive nature of the procedures involved. Understanding these risks is vital for implementing effective countermeasures:</p><ul style="list-style-type: disc;"><li><p>Cross-Contamination</p></li></ul><p>Handling multiple samples can simultaneously lead to cross-contamination if equipment and surfaces are not properly sanitized between procedures.</p><ul style="list-style-type: disc;"><li><p>Environmental Contaminants</p></li></ul><p>Airborne particles, microbial contamination, and even previously amplified DNA can introduce nucleic acid contaminants into the sample.</p><ul style="list-style-type: disc;"><li><p>Reagent Contamination</p></li></ul><p>Reagents used in extraction processes can be contaminated with DNA or RNA from their manufacturing environment, potentially leading to false results.</p><ul style="list-style-type: disc;"><li><p>Human Error</p></li></ul><p>Mishandling of samples, improper use of equipment, and failure to follow protocols meticulously can all introduce contaminants into the extraction process.</p><p><strong>Strategies to Minimize Lab Contamination</strong></p><p>To safeguard the integrity of DNA and RNA extraction processes, several proactive strategies can be employed:</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20240619/1718780234596744.png" title="" alt="2.png"/></p><ul style="list-style-type: disc;"><li><p>Rigorous Protocol Adherence</p></li></ul><p>Ensuring that all laboratory personnel are trained in and strictly adhere to standardized protocols can significantly reduce human error.</p><ul style="list-style-type: disc;"><li><p>Use of Barrier Methods</p></li></ul><p>Implementing physical barriers, such as gloves and face masks, and using dedicated tools for different stages of the extraction process can help prevent cross-contamination.</p><ul style="list-style-type: disc;"><li><p>Regular Equipment Maintenance and Calibration</p></li></ul><p>Keeping all laboratory equipment well-maintained and regularly calibrated minimizes the risks of mechanical errors leading to contamination.</p><ul style="list-style-type: disc;"><li><p>Quality Control Measures</p></li></ul><p>Regularly testing reagents for contamination and employing negative controls in extraction runs can help identify and mitigate contamination sources promptly.</p><ul style="list-style-type: disc;"><li><p>Employment of Enclosed Automated Systems</p></li></ul><p>Utilizing automated nucleic acid extraction systems that operate within a closed environment can drastically reduce the exposure of samples to potential contaminants.</p><p>By integrating these strategies into daily laboratory operations, researchers could lower the chances of lab contamination. It improves the reliability and accuracy of their DNA and RNA extraction results, ensuring that their findings are both valid and reproducible.</p><p><strong>Introducing Tianlong's GeneRotex 96 Nucleic Acid Extractor</strong></p><p><a href="https://www.medtl.net/products/generotex-96-nucleic-acid-extractor.html" target="_self">Tianlong's GeneRotex 96 Nucleic Acid Extractor</a> represents a significant advancement in the field of automated nucleic acid extraction, offering a host of features designed to minimize lab contamination. This nucleic acid extraction machine is viably engineered to handle the complexities of DNA and RNA extraction with precision and reduced risk of lab contamination. Here are the key features of this automatic nucleic acid extraction machine:</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20240619/1718780254816636.png" title="" alt="3.png"/></p><ul style="list-style-type: disc;"><li><p>RMT Technology</p></li></ul><p>A distinguishing feature of the GeneRotex 96 Nucleic Acid Extractor is its use of self-developed Rotational Mixing Technology (RMT). Traditional oscillation-based mixing methods are prone to generating aerosols, which can carry nucleic acids and other contaminants, posing a substantial risk for cross-contamination. The RMT technology equipped at GeneRotex 96 Nucleic Acid Extractor reduces aerosol generation by over 50% compared to conventional techniques. By minimizing aerosol production, the risk of cross-contamination is significantly lowered, thereby enhancing the accuracy and reliability of experimental results.</p><ul style="list-style-type: disc;"><li><p>Advanced HEPA Filtration and Negative Pressure System</p></li></ul><p>The GeneRotex 96 Nucleic Acid Extractor incorporates an advanced HEPA filtration system in conjunction with a negative pressure airflow mechanism when doing DNA and RNA extraction. The built-in HEPA filter is highly efficient in capturing airborne particles, including potential contaminants. The negative pressure system ensures that expelled air is effectively filtered, greatly reducing the risk of environmental contamination.</p><p>This dual-layer protection system not only maintains the integrity of the samples but also curbs the chances of lab contamination, enhancing the safety of personnel and other experiments.</p><ul style="list-style-type: disc;"><li><p>Integrated UV Disinfection</p></li></ul><p>The GeneRotex 96 Nucleic Acid Extractor features integrated UV disinfection capabilities. Equipped with UV lamps, this automatic nucleic acid extraction system can be programmed to disinfect the working area before and after each use. UV disinfection is a well-established method for creating a sterile environment for DNA and RNA extraction, providing additional protection against contamination in the lab.</p><ul style="list-style-type: disc;"><li><p>Automated Door System</p></li></ul><p>Human error is a common source of contamination in laboratory settings. The GeneRotex 96 Nucleic Acid Extractor addresses this issue with its automated door system, which uses a motor-driven component to open and close the chamber doors without manual handling. This reduces the risk of contamination from human contact with potentially contaminated surfaces as handling the DNA and RNA extraction tasks.</p><ul style="list-style-type: disc;"><li><p>Other Features</p></li></ul><p>Except for advanced disinfect measure and automatic features to complete the DNA and RNA extraction better, here are more features of GeneRotex 96 Nucleic Acid Extractor to present the fast, high-capacity, and reliable nucleic acid result:</p><p>1. High Throughput and Efficiency: Capable of handling anywhere from 1 to 96 samples per run, the GeneRotex 96 Nucleic Acid Extractor accommodates 96 deep-well plates and six tube strips. This design ensures high throughput while reducing reagent waste, optimizing both resource usage and experimental scalability.</p><p>2. Built-in Full-Color Touch Screen:The inclusion of a 7-inch color LCD touch screen makes the system user-friendly and simplifies the operation of complex experiments.</p><p>3. High Purification: With less than 1% residual magnetic beads after purification, the GeneRotex 96 Nucleic Acid Extractor delivers high-purity nucleic acids, which is essential for reliable experimental results.</p><p>These innovative features make the Tianlong GeneRotex 96 Nucleic Acid Extractor an essential tool for laboratories where they seek effectiveness and efficiency of the nucleic acid extraction techniques while significantly reducing the potential for lab contamination.</p><p><strong>Conclusion</strong></p><p>Minimizing lab contamination during DNA and RNA extraction is crucial for achieving reliable and accurate molecular research outcomes. By understanding the possibility of contamination and implementing robust preventive strategies, researchers can significantly enhance the integrity of their experimental results. The adoption of advanced machines, like Tianlong's GeneRotex 96 Nucleic Acid Extractor, plays a pivotal role in this process. With features such as advanced EMT technology, HEPA Filtration, high-throughput capabilities, and more, the GeneRotex 96 Nucleic Acid Extractor sets a new standard in minimizing contamination risks.</p><p>This nucleic acid extraction machine is also an embodiment of Tianlong's extensive experience and commitment to innovation in the molecular study industry, positioning us as a trusted leader. By choosing us, Tianlong, researchers not only invest in superior technology but also gain a partner renowned for its expertise and reliability in facilitating groundbreaking molecular discoveries. You can visit <a href="https://www.medtl.net/" target="_self">Tianlong</a>’s official website to learn more.</p>" ["url"] => string(88) "/news/blog/how-to-reduce-chances-of-lab-contamination-during-dna-and-rna-extraction.html" ["count"] => string(3) "346" ["sort"] => string(1) "0" ["command"] => string(1) "0" ["isdel"] => string(1) "0" ["free"] => string(1) "0" ["status"] => string(1) "1" ["uid"] => string(1) "4" ["username"] => string(11) "system-2022" ["euid"] => string(1) "4" ["edituser"] => string(11) "system-2022" ["input_time"] => string(10) "1718779969" ["create_time"] => string(10) "1718780356" ["update_time"] => string(10) "1719985096" ["static_title"] => string(0) "" ["tag"] => NULL ["out_url"] => string(0) "" ["custom_code"] => string(0) "" ["jie"] => string(185) "This article delves into practical strategies to mitigate contamination risks during the crucial stages of DNA and RNA extraction, ensuring more reliable and accurate research outcomes." ["is_ding"] => string(1) "0" ["zname"] => string(0) "" ["index_img"] => array(2) { ["thumb"] => string(52) "/Public/Uploads/uploadfile/images/20240619/2-191.jpg" ["alt"] => string(9) "2-191.jpg" } ["vid"] => string(0) "" ["command_detail"] => string(1) "0" ["title_url"] => string(1) "1" ["url_title"] => string(1) "1" ["iframe"] => string(0) "" }
This article delves into practical strategies to mitigate contamination risks during the crucial stages of DNA and RNA extraction, ensuring more reliable and accurate research outcomes.
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2024-05-13Portable PCR Machine: How Does It Empower Veterinarians to Test Animal Virus?
array(34) { ["id"] => string(3) "608" ["catid"] => string(3) "498" ["title"] => string(77) "Portable PCR Machine: How Does It Empower Veterinarians to Test Animal Virus?" ["thumb"] => array(2) { ["thumb"] => string(76) "/Public/Uploads/uploadfile/images/20240513/weixinjietu_20240513110541(1).png" ["alt"] => string(33) "weixinjietu_20240513110541(1).png" } ["keywords"] => string(0) "" ["description"] => string(0) "" ["content"] => string(11367) "<p>Veterinarians are constantly vigilant about the threat of animal viruses. The fear is twofold: first, the direct impact on animal health, where viruses can swiftly spread through herds or flocks, causes illness and death. Second, the possibility of these viruses crossing species barriers and infecting humans leads to potential public health crises.</p><p>Thus, rapid identification and containment are paramount in preventing widespread outbreaks. One efficient tool for modern veterinarians is portable Polymerase Chain Reaction machines or <a href="https://www.medtl.net/products/instruments/real-time-pcr-systems" target="_self">portable PCR machines</a>. These devices have transformed the landscape of disease detection and control by allowing veterinarians to identify animal viruses rapidly and accurately in the field.</p><p><br/></p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20240513/1715569569728144.png" title="" alt="微信截图_20240513110541.png"/></p><p style="text-align: center;"><span style="font-size: 12px;"><em>(Copyright photo from https://www.freepik.com/free-photo/medium-shot-doctor-checking-dog_22881474.htm#fromView=search&page=1&position=10&uuid=22b74e92-0544-4557-b1c4-6a055b3dd0e3)</em></span></p><p><br/></p><p><strong>Common Animal Viruses</strong></p><p>In recent years, several common animal viruses have spread globally, impacting different species:</p><p><br/></p><p>Ø Virus in Dogs</p><p>There has been a notable outbreak of a canine respiratory disease, which is part of the canine infectious respiratory disease complex (CIRDC). This syndrome can be caused by various bacterial and viral pathogens and is highly contagious. This animal virus has been reported in at least 10 states in the U.S., with cases estimated in the thousands. The clinical signs are often mild but can progress to severe disease and death. This has led to challenges in developing a definitive test and in implementing targeted treatment.</p><p>Ø Virus in Swine</p><p>Recent global surveillance has highlighted the prevalence of several viruses in the swine population, with significant implications for animal health and potential zoonotic transmission. The African Swine Fever Virus (ASFV) continues to be a major concern, with ongoing research into gene-deleted live-attenuated vaccines showing promise for protecting pigs against this highly lethal disease. Additionally, other viruses such as Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Porcine Epidemic Diarrhea Virus (PEDV) are subjects of intensive study due to their economic impact on the swine industry and their potential to cause severe disease in pigs. The genetic diversity and evolution of these animal viruses are critical areas of focus to enhance the understanding and develop effective strategies for disease management and prevention.</p><p>Ø Virus in Birds</p><p>While the search results do not provide specific recent outbreaks in birds, avian influenza or bird flu, particularly the H5N1 and H7N9 subtypes, has been a recurring concern worldwide. These viruses can not only cause severe disease in birds but also pose a zoonotic risk, infecting humans with serious health implications. Additional viruses of concern in birds include infectious bronchitis virus (IBV) in chickens, which leads to significant economic losses in the poultry business due to reduced egg production and growth inhibition in chickens.</p><p><br/></p><p><strong>Solutions to Detect Animal Virus</strong></p><p>For veterinarians, the choice of diagnostic testing method is critical in managing animal health and disease outbreaks. Here's a closer look at the pros and cons of each method from a veterinary perspective:</p><p>1. Viral Culture</p><p>It is the traditional method for confirming the presence of a live virus, which is beneficial for animal virus research and vaccine development, including its growth requirements and susceptibility to antiviral drugs.</p><p>However, viral culture is a time-consuming process, often taking days to weeks, which can delay diagnosis and treatment. Plus, it requires specialized laboratory facilities and trained personnel. And there is a risk of exposure to the pathogen for laboratory staff, which may not be available in all veterinary clinics.</p><p>2. Anti-Body Testing</p><p>Antibody tests are valuable for determining past exposure to a virus within a population, offering insights into the immune status and seroepidemiology. These tests are generally less expensive and provide quicker results than other diagnostic methods, making them a cost-effective option for large-scale surveys.</p><p>But this solution cannot confirm active infections and may not distinguish between different strains of a virus. Additionally, the presence of antibodies does not always equate to protective immunity, which can complicate the interpretation of results.</p><p>3. Antigen Testing</p><p>Antigen testing detects the presence of viral proteins, which can indicate an active infection. This method offers a relatively rapid means of diagnosis compared to viral culture and can be adapted for point-of-care testing, which is beneficial for quick decision-making.</p><p>However, antigen tests are often less sensitive than nucleic acid tests, leading to a higher risk of false negatives, especially when the viral load is low, as in the early or late stages of infection.</p><p>4. Nucleic Acid Testing</p><p>Although the cost of nucleic acid testing (NAT) would be higher than other solutions, NAT is highly sensitive and specific, making it the gold standard for detecting viral nucleic acids.</p><p>NAT is a type of diagnostic test that detects the genetic material of viruses or other pathogens, such as DNA or RNA. It can identify the animal virus at low levels, even before the onset of clinical symptoms, which is crucial for early detection and intervention. Besides, the NAT allows for the differentiation of virus strains and the monitoring of genetic changes, providing valuable information for tracking outbreaks and understanding transmission dynamics.</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20240513/1715569701418345.png" title="" alt="微信截图_20240513110718.png"/></p><p><br/></p><p><strong>Introduction of Portable PCR Machine</strong></p><p>The Polymerase Chain Reaction (PCR) machine is a critical tool used to perform NAT. For veterinarians, the PCR machine is one of the effective tools to have PCR testing for dogs, swine, domestic fowl, or other animals. It can effectively detect the animal virus to prevent and control the spread of animal viruses and deliver the clinical solution. Among various kinds of PCR machines, the portable one is suitable for veterinarians to detect animal viruses. Talking specifically, here’s why:</p><p>Timely Intervention</p><p>Early detection of a viral outbreak is critical in controlling its spread. Portable PCR machines allow for faster identification of pathogens, which can lead to quicker interventions, reducing the impact on animal health and the potential for economic losses.</p><p>Sample Integrity Assurance</p><p>A portable PCR machine can ensure sample integrity for veterinarians. Samples analyzed immediately after collection are less likely to be compromised by degradation, ensuring that the genetic material remains intact and the results are as accurate as possible. Besides, the elimination of transport delays accelerates the diagnostic process, allowing for faster identification of diseases and immediate implementation of control measures, which is essential in managing outbreaks and preventing further spread.</p><p>Accessibility in Remote Locations<br/></p><p>Traditional PCR equipment is often large and requires a stable power supply and temperature-controlled environments, which may not be available in remote or rural areas. A portable PCR machine, with its lightweight and compact design, can be easily carried and operated in these challenging environments, empowering veterinarians with diagnostic capabilities where they are needed most.</p><p>Reasons to Choose Tianlong’s Gentier Mini+ 4 Channels Portable Real Time PCR System</p><p>Veterinarians, entrusted with the health and welfare of animal patients, must meticulously select diagnostic tools that embody precision, efficiency, and versatility. Our <a href="https://www.medtl.net/products/gentier-mini-4-channels-portable-real-time-pcr-system.html" target="_self">Tianlong's Gentier Mini+ 4 Channels Portable Real-Time PCR System</a> is a reliable portable PCR machine that is tailored to meet the demands of veterinary diagnostics:</p><p>Optimized Efficiency</p><p>Time is precious in veterinary practice. Otherwise, it would render animal virus outbreaks and a sum of economic loss. The Gentier Mini+ 4 Channels Portable Real-Time PCR System's remarkable capability to complete all 16 wells of fluorescence scanning within a mere second is a testament to its unrivaled efficiency. Through the integration of dual fluorescence channels, our portable PCR machine streamlines workflow processes, enabling veterinarians to expedite sample analysis without compromising accuracy.</p><p>Robust Analytical Capabilities</p><p>The Gentier Mini+ 4 Channels Portable Real-Time PCR System is a comprehensive and professional analytical tool for the detection of pathogens. Equipped with sophisticated software, our digital PCR system can empower veterinarians with a diverse array of analytical functions, ranging from qualitative to quantitative analyses. Whether discerning subtle genetic variations through SNP analysis or quantifying target sequences with absolute precision, this PCR machine affords veterinarians unparalleled insights into the molecular landscape of their patients.</p><p>Practical Portability</p><p>Adaptability means efficiency when the animal virus outbreaks or becomes an outbreak. Our Gentier Mini+ 4 Channels Portable Real-Time PCR System's compact dimensions (205mm(L)*156mm(W)*153mm(H)) and featherweight construction (3.2kg) epitomize practicality, conserving valuable bench space while facilitating seamless mobility. Its portability empowers veterinarians to conduct on-site testing in mobile laboratories with effortless ease. Moreover, the system's innate stability obviates the need for fluorescence recalibration post-relocation, ensuring uninterrupted diagnostic precision regardless of setting.</p><p><br/></p><p><strong>Conclusion</strong></p><p><br/></p><p>For veterinarians, resorting to NAT is one of the effective solutions to prevent and control the animal virus outbreak. Aims to respond fast and find out the pathogeny, our Tianlong’s Gentier Mini+ 4 Channels Portable Real-Time PCR System is an efficient tool for its compactness, fast real-time testing, and analytics ability. And we, <a href="https://www.medtl.net/" target="_self">Tianlong</a>, are a professional manufacturer of nucleic acid testing instruments and reagents with over 20+ experience. You can browse our website to learn more information about our digital PCR machines and <a href="https://www.medtl.net/contact" target="_self">contact us</a> easily. </p>" ["url"] => string(91) "/news/blog/portable-pcr-machine-how-does-it-empower-veterinarians-to-test-animal-virus.html" ["count"] => string(3) "382" ["sort"] => string(1) "0" ["command"] => string(1) "0" ["isdel"] => string(1) "0" ["free"] => string(1) "0" ["status"] => string(1) "1" ["uid"] => string(1) "4" ["username"] => string(11) "system-2022" ["euid"] => string(1) "4" ["edituser"] => string(11) "system-2022" ["input_time"] => string(10) "1715569479" ["create_time"] => string(10) "1715569808" ["update_time"] => string(10) "1721099221" ["static_title"] => string(0) "" ["tag"] => NULL ["out_url"] => string(0) "" ["custom_code"] => string(0) "" ["jie"] => string(117) "One efficient tool for modern veterinarians is portable Polymerase Chain Reaction machines or portable PCR machines. 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One efficient tool for modern veterinarians is portable Polymerase Chain Reaction machines or portable PCR machines.
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